PhD in Engenharia Agronómica, ISA, Technical University of Lisbon
Master in Biotechnology and Biochemical Engineering, IST - Technical University of Lisbon
Degree in Chemistry, Faculty of Sciences - Lisbon University

 “Professor-Adjunto”. Teacher of the disciplines Laboratory I and Laboratory II, no curso de Licenciatura Bi-etápica de Turismo, Hotelaria e Termalismo do Instituto Superior de Ciências Educativas em Ramada-Odivelas.

Teacher of the disciplines Físico-Química de Cursos Profissionais de Nível III, (95 h), Instituto de Tecnologias Náuticas, Paço d’Arcos.


 Equiparada a Assistente do 1º Triénio. Teacher of the disciplines: Biochemistry, Chemistry, Technology of the Animal Products I and Food Chemistry. Escola Superior Agrária de Santarém. Instituto Politécnico de Santarém

Temporary appointment as teacher of the disciplines: Biochemistry, Environment Sciences and Laboratory Techniques in Chemistry. Secondary School of Parede. Portugal

Graduate student in the projects from FCT “Proteínas de L. albus que intervêm no desenvolvimento e na reacção a agentes patogénicos” (PRAXIS XXI 2/2.1/BIO/1146) and “Role of proteases and their inhibitors in the Lupinus albus responses to drought” (Centro de Botânica Aplicada à Agricultura, 2002-03) aiming the detection, purification and molecular characterization of novel small metalloproteinase protein inhibitors by utilizing ionic exchange, gel filtration, reverse-phase and affinity chromatographies, spectrophotometric and spectrofluorimetric assays, mono and bidimensional electrophoresis.
Laboratório de Bioquímica Vegetal, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras e Departamento de Botânica e Engenharia Biológica, Instituto Superior de Agronomia, Universidade Técnica de Lisboa

Graduate student in the project from CE (Human Capital and Mobility CT94-0535) on “Innovative Analysis and Engineering of proteolytic Enzymes and Their Inhibitors” aiming the study of proteases and their inhibitors utilizing chromatographies (HPLC) , spectrophotometric and spectrofluorimetric assays electrophoretic and radiometric analysis. Laboratory of Biochemistry II. Gulbenkian Institute of Science (Oeiras) Portugal

Temporary graduate student in the project from CE (Human Capital and Mobility CT94-0535) on “Innovative Analysis and Engineering of proteolytic Enzymes and Their Inhibitors” aiming kinetic studies with spectrofluorimetric techniques. Abteilung für Klinishe Biochemie (Universitat München) Deutchland

Temporary graduate student in the project “Biotechnology of Extremophyles” sponsered by EC (BIOTECH) aiming the study of the optimal growth conditions of sulphur-dependent bacteria. Department of Biochemistry and Biophysics. ITQB (New University of Lisbon)

Undergraduate student. Project on “Indirect immunofluorescence as a confirmatory test on HIV infection diagnosis” aiming the evaluation of the sensitivity and specificity of indirect immunofluorescence for HIV infection diagnosis utilizing antibodies and antigens search techniques, processing of human blood, immunoenzimatic assays, nucleic acid extraction techniques, PCR and confocal fluorescence microscopy. Department of Immunology. Faculty of Medical Sciences (New University of Lisbon) Classification: Excellent (95%)

Search for novel natural compounds from plants with potential biomedical applications;
Kinetic studies of protease inhibitory activities;
Cellular biology and proteomic analysis of animal cell cultures

Articles in international journals

Dina M. Carrilho, Isabel C. Duarte, Rita Francisco, Cândido P. P. Ricardo, Maria C. Duque-Magalhães (submitted to the Biochimica et Biophysica Acta) “Proteomic analysis of new peptides with metalloproteinase inhibitory activity”

Carrilho, D. M. P., Duque-Magalhães, M. C. (in preparation to be submitted to the Journal of Biological Chemistry) “Novel metalloproteinase peptide inhibitor. I - Purification and characterization of a 6.5 kDa peptide”

Carrilho, D. M. P., Duque-Magalhães, M. C. (in preparation to be submitted to the Journal of Biological Chemistry) “Novel metalloproteinase peptide inhibitor. II -«Purification and characterization of a 3 kDa peptide”

Carrilho, D. M. P., Duque-Magalhães, M. C. (in preparation) “New plant peptide inhibitor of thermolysin”

Alves, M., Carrilho, D. M. P., Chaves, I., Ricardo, C.P.P. (in preparation “Combined two-dimensional electrophoresis and reverse zymography for the isolation of new trypsin inhibitors with lectin domains in Phaseolus vulgaris (bean) seeds”

Thesis

Carrilho, D.M.P. (2006) “Purification and molecular characterization of novel small metaloproteinase protein inhibitors in Lupinus albus quiescent seeds“,I.S.A., Technical University of Lisbon, Lisbon.

Carrilho, D.M.P. (1997) “Identification, isolation and partial characterization of novel protein inhibitors of metalloproteinases in Lupinus albus seeds”, I.S.T., Technical University of Lisbon, Lisbon.

Carrilho, D.M.P. (1993) “Indirect immunofluorescence as a confirmatory test on HIV infection diagnosis”, Faculty of Sciences, Lisbon University, Lisbon.

Oral Communications by invitation

Inês Chaves, Dina Carrilho, Marta Alves, Conceição Duque-Magalhães, Ana P. Regalado, Cândido P. Ricardo (2004) “Analysis of the protease activity during programmed cell death induced in nicotiana tabacum suspension-cultured cells by (beta-d-galactosyl3yariv reagent” XIV Congresso Nacional de Bioquímica, Tivoli marinotel, Vilamoura.

Carrilho, D. M. P. and Duque-Magalhães, M. C. (2003) "The human matrix-metalloproteinase-type 9 is strongly inhibited by small peptides from the lupin seed” I Luso-Spanish Workshop on the Structure and Function of Proteins, Faculdade de Informática, Campus de Elviña, Universidade de A Coruña, Spain.

Carrilho, D. M. P.; C. P. P. Ricardo; Duque-Magalhães, M. C. (2002) “Thermolysin-affinity chromatography for purification of protein protease inhibitors from the lupin seed”. XIII Congresso Nacional de Bioquímica. Lisbon.

Carrilho, Dina M. P. and Duque-Magalhães, Maria C. (1999) "LASI-1 and LASI-2: Two novel metalloproteinase protein inhibitors of small molecular mass". International Proteolysis Society First General Meeting. Mackinac Island, Michigan, USA.

Carrilho, Dina M. P. and Duque-Magalhães, M. Conceição (1998) "Proteinaceous metalloproteinase inhibitors from seeds". XI Congresso Nacional de Bioquímica. Tomar. Portugal.

Carrilho, D. M., Abreu, I. and Barros, M. F. (1994) “Subcellular localization of antigens visualised on 8E5/LAV cells by sera from human immunodeficiency virus (HIV) patients”. XX Meeting of the Portuguese Society of Immunology. Lisbon. Portugal